Characterization, Genome Analysis and Probiotic Properties of L-Lactic Acid Producing Enterococcus lactis FM11-1

Strain FM11-1 was isolated from flowers of Solanum torvum that were collected in Nakhon Si Thammarat Province, Thailand and characterized using a polyphasic approach as Gram-positive, facultatively anaerobic with short chains catalase-negative cocci. This strain produced L-lactic acid glucose closely related to Enterococcus durans NBRC 100479T, faecium NRIC 1145T, hirae ATCC 9790T, lactis LMG 25958T ratti DSM 15687T (98.92-99.73 %) based on 16S rRNA gene sequence similarity. The draft genome had 2,784,928 bp contained 2,586 coding sequences, genomic G+C content 38.07 mol%. Values ANIb, ANIm digital DNA-DNA hybridization (dDDH) between the closest strain, E. 97.23%, 98.30% 83.7%, respectively. predominant cellular fatty acids C19:0 cyclo 8c C16:0. identified characterization analysis. genes involved carbohydrate fermentation specialty antibiotic resistance. showed adhesion ability (0.43±0.11) Caco-2 cells but no cytotoxicity against Caco-2, HepG2 Vero cells.